Not if you only select option 2, that will use the old boot.wim registry mods. But this is @AveYo his thread, will continue this in my own tool thread.
Thanks to AveYo, a v11 of Skip_TPM_Check_on_Dynamic_Update is up on GitHub. It accounts for the new build scheme (25xxx for Canary, 23xxx Dev, 22000 Beta).by updating what I think is the RegEx pertaining to build numbers.
Just to be clear: I think (emphasis on "think") that if you write an ISO image to a flash drive with Rufus, and then run setup.exe from the flash drive, the upgrade should work?
Upgrades don't need bootable install media, just mount or extract the ISO to a local folder and run setup.exe, apply fixes to the ISO or first run dynamic tpm skip script from this thread.